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Daily medium change


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3 replies to this topic

#1 Biophysics

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Posted 24 January 2010 - 07:38 AM

Hi,
if i changed the medium every day, do i need for co2 incubator?
please answer me, i am new in stem cell culture.

#2 Biophysics

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Posted 26 January 2010 - 09:42 AM

pleeeeease answer me.

#3 euwing

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Posted 05 February 2010 - 02:14 PM

which stem cells are you culturing? If those are human embryonic stem cell, they are very sensitive so you have to use incubator with CO2 and temperature control. In fact, I would put all my cell cultures in the incubator.

#4 biocloners

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Posted 02 March 2010 - 08:31 PM

Hi,
if i changed the medium every day, do i need for co2 incubator?
please answer me, i am new in stem cell culture.


I think u still need CO2 incubator, because it's so important to stabilize the pH medium, especially if your medium contain Sodium Bicarbonate.
Maybe it useful for you :P




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