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Centrifuge after lysing


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5 replies to this topic

#1 Halfro22

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Posted 23 January 2010 - 09:29 AM

I have seen in many protocols what samples will be centrifuged after they are lysed. What is the purpose of this? I can't seem to find the answer anywhere!

#2 bob1

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Posted 24 January 2010 - 03:34 PM

to pull down the chunks of un-dissolved and insoluble material left behind from the cells lysing. If left in suspension, it will make aliquoting and pipetting of your samples difficult.

#3 swanny

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Posted 24 January 2010 - 09:25 PM

... chunks of un-dissolved and insoluble material ...

Good to see your grasp on the finer points of technical language is still up there, bob :D :lol:
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.

#4 bob1

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Posted 25 January 2010 - 05:22 PM

Thanks,

Just trying to be the common person these days ;)

#5 ursae

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Posted 18 July 2010 - 06:10 PM

hi, i use a very strong sds to lyse my cells and i usually do not centrifuge. and it works well as my target proteins is inside the nucleus. Any account for that?

#6 bob1

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Posted 19 July 2010 - 01:49 PM

IF it works, then there probably isn't a problem. You may find that your cultures have a few lumps of stuff in them occasionally that makes pipetting hard.




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