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hybridization using fluorescein-12-UTP


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#1 aztecan princess

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Posted 21 January 2010 - 04:24 PM

We plan to do some labelled probes for In situ hybridization using fluorescein-12-UTP (Fluorescein RNA Labeling Mix from roche).
I知 not sure if the probe値l fluoresce by itself or if it has to be detected using a conjugated anti-fluoorescein AP (or other). So, do I need to acquire the anti-fluorescein?

Thanks for your help! :)





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