DAB & DAPI together for ihc?
Posted 21 January 2010 - 12:53 PM
Posted 21 January 2010 - 03:25 PM
Posted 22 January 2010 - 08:37 AM
Posted 24 January 2010 - 03:32 PM
Posted 13 January 2017 - 11:05 PM
Have you tried and how was that?
for me, dapi(vectashield) and cfos-DAB didn't go well together... where dab stained seen dark in dapi.
maybe dab pigment opaqued dapi emission light for they stain cellular nucleus(because rather for it was cfos than dab itself) together
Edited by miza, 13 January 2017 - 11:07 PM.
Posted 15 November 2017 - 03:09 AM
Insofar as the DAB won't autofluoresce or meddle with the DAPI fluorescence, at that point it ought to be OK. Know that intensely settled tissue (e.g. formaldehyde settled) has a great deal of foundation fluorescence because of the aldehydes abandoned.