Postive mRNA Control Following 5-Aza-dc treatment
Posted 21 January 2010 - 06:40 AM
Posted 23 February 2010 - 12:38 PM
So I have treated some cells with 5 Aza dc and am looking for a good imprinted gene that normally is repressed in terminally differentiated fibroblasts. Any good ideas out there?
So I did my 5aza dC experiments by using the H19 DMR as a control for loss in DNA methylation and it works pretty well using primary human fibroblast cell line.
Edited by LucasRudy, 23 February 2010 - 12:38 PM.