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Postive mRNA Control Following 5-Aza-dc treatment


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#1 racingstud

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Posted 21 January 2010 - 06:40 AM

So I have treated some cells with 5 Aza dc and am looking for a good imprinted gene that normally is repressed in terminally differentiated fibroblasts. Any good ideas out there?

thanks, Matt.

#2 LucasRudy

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Posted 23 February 2010 - 12:38 PM

So I have treated some cells with 5 Aza dc and am looking for a good imprinted gene that normally is repressed in terminally differentiated fibroblasts. Any good ideas out there?

thanks, Matt.


Hey Matt,

So I did my 5aza dC experiments by using the H19 DMR as a control for loss in DNA methylation and it works pretty well using primary human fibroblast cell line.
cheers

Edited by LucasRudy, 23 February 2010 - 12:38 PM.





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