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how to prepapre 2mM 8-Hydroxyquinoline for root tip treatment


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#1 peixumol

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Posted 21 January 2010 - 03:42 AM

Dear all,
I am going to do FISH in some day, the protocol for which includes preparing 2mM 8-Hydroxyquinoline to treat soybean root tips. However, 8-Hydroxyquinoline is water insoluble, neither I found it completely disovled in ethonal at 2mM at room temp. could anyone experienced tell me how to prepare 2mM 8-Hydroxyquinoline correctly?
Many many thanks in advance.
paul in NanJing China
God helps them who help themselves!

#2 mdfenko

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Posted 21 January 2010 - 09:13 AM

according to the merck index (9th edition), 8-hydroxyquinoline is freely soluble in alcohol, acetone, chloroform, benzene, and aqueous mineral acids.

it should have dissolved in ethanol. did you use absolute or diluted ethanol?
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#3 peixumol

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Posted 21 January 2010 - 09:33 PM

according to the merck index (9th edition), 8-hydroxyquinoline is freely soluble in alcohol, acetone, chloroform, benzene, and aqueous mineral acids.

it should have dissolved in ethanol. did you use absolute or diluted ethanol?



Hi friend,
I used absolute ethanol. Now I doubt whether the problems is the purity of 8-hydroxyquinoline I used? your opinion?
Thanks deeply.

Pei
paul in NanJing China
God helps them who help themselves!

#4 mdfenko

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Posted 22 January 2010 - 09:13 PM

it could be impure preparation. what does the analysis sheet say?

how old is the compound? maybe some moisture got in and caused it to cake? that would make it difficult to dissolve.
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genius does what it must
i do what i get paid to do

#5 peixumol

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Posted 24 January 2010 - 11:46 PM

it could be impure preparation. what does the analysis sheet say?

how old is the compound? maybe some moisture got in and caused it to cake? that would make it difficult to dissolve.

Yes,I think so.
Thanks a lot!
Cheers!
paul in NanJing China
God helps them who help themselves!




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