4 replies to this topic

[mustyimam]

Can anyone please tell me how much blood volume a rat has based  on its weight? and how much of it can i withdraw while still experimenting (mid-way) without causing any serious hemodynamic instability? thanx

[aimikins]

if you go to google and type: "blood volume in a rat?" several promising links appear.

[Stace]

This is generally the rule that our lab follows, and it seems to be the norm.

The 1-3-6 rule is generally followed, in that the average blood volume of most laboratory animals is 6% body weight (60 ml/kg). The most blood that can be expected from a terminal sacrifice is 3% body weight (30 ml/kg); and no more than 1% (10 ml/kg) body weight should be collected during any 2-week period from animals surviving the blood collection.

I forget where I found that rule. It had to be on an SOP of a University animal facility of some sort. Hope that helps out a bit.

Stacy

[mustyimam]

aimikins, on Jan 22 2010, 02:57 AM, said:

if you go to google and type: "blood volume in a rat?" several promising links appear.

Stace, on Jan 22 2010, 06:06 AM, said:

This is generally the rule that our lab follows, and it seems to be the norm.

The 1-3-6 rule is generally followed, in that the average blood volume of most laboratory animals is 6% body weight (60 ml/kg). The most blood that can be expected from a terminal sacrifice is 3% body weight (30 ml/kg); and no more than 1% (10 ml/kg) body weight should be collected during any 2-week period from animals surviving the blood collection.

I forget where I found that rule. It had to be on an SOP of a University animal facility of some sort. Hope that helps out a bit.

Stacy

Thanx alot guy.

[karljoesen]

well i think brat have many specific brat so you can't have an accurate figures.