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Protein Standard stability (BCA assay)


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#1 sitizhassan

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Posted 20 January 2010 - 06:56 AM

Hi
Does anyone know if the standard protein for BCA assay left out in the room temperature for a month, will it maintain the stability? It was my first time buying that BCA assay kit. I did not aware that the standard must be kept in the 2-8 degree C. Is it possible for me to use it for my assay?

#2 mdfenko

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Posted 20 January 2010 - 07:02 AM

if it was rehydrated then it is probably shot. if not then you should determine if you can get it all into solution. if not then it is shot.

best to buy a fresh standard (shouldn't be too expensive).
talent does what it can
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#3 sitizhassan

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Posted 20 January 2010 - 08:49 AM

if it was rehydrated then it is probably shot. if not then you should determine if you can get it all into solution. if not then it is shot.

best to buy a fresh standard (shouldn't be too expensive).


thanks. i will try it first, if it doesn't give a reliable result, i'll get the new one.

#4 Prep!

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Posted 21 January 2010 - 03:34 AM

Hey there.... corerct me if i am wrong.... if u using the pierce (Thermo) kit for BCA.. the standard supplied with it is BSA... which is liquid and the storage temperature is Room temepature!!!! so i see no reason to worry as long as u are not using the protein of your intereest as a standard!!! :)

Edited by Pradeep Iyer, 21 January 2010 - 03:35 AM.

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