Posted 20 January 2010 - 04:27 AM
Posted 03 February 2010 - 07:32 AM
I haven't been able to design primers yet. But the following might be good lecture to start with.
Methods Mol Biol. 2009;507:305-23.
Licchesi JD, Herman JG.
Cancer Biology Program, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins, Baltimore, MD, USA.
Posted 17 February 2010 - 09:37 PM
First PCR using Universial primers - only amplify modified DNA
2nd PCR using M primers to amplify methylation DNA, U primers to amplify unmethylated DNA.