cDNA concentration higher than original RNA
Posted 19 January 2010 - 07:25 PM
my RNA has 1.8 ng/ul concentration but the cDNA that I made from it has 1.2 ug/ul concentration. Even higher.
How is this possible when I used only 2 ul of RNA as tamplate in 20 ul reaction. I mean the cDNA concentration must be even lower, but now it is higher. How?
Posted 20 January 2010 - 01:48 AM
Posted 20 January 2010 - 08:21 AM
Posted 17 February 2010 - 11:44 PM