Posted 19 January 2010 - 02:51 PM
Posted 19 January 2010 - 09:28 PM
You could establish an immunometric (eg sandwich ELISA) assay for mouse immunoglobulin:
We are devel :Do ping monoclonal antibodies. We would like to quantitate the supernatant before doing the SIB assay as some of the sups turn up negative due to low concentration, but I fear this isn't an option. Does anyone have any ideas??
Solid phase: goat anti-mouse light chains
Tracer: HRP goat anti-mouse Fc
Calibrate using standards made from a mouse monoclonal IgG.
You could use polyclonal goat anti-mouse as both solid phase and tracer. Crude but should work fine for your purpose.
Hope this helps
Posted 20 January 2010 - 08:42 AM