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How Much BrET?


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#1 sorcener

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Posted 19 January 2010 - 10:40 AM

Hi everybody!

Just a small question, I want to reduce BrET use in my experiments and as we have only 1 microware used also for other people and things i prefer to use BrET on my TAE running buffer instead on gel.

In your experience wich is the smallest concentration of BrET I can use on my buffer in order to have a good DNA view?

Thanks a lot!

#2 molgen

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Posted 20 January 2010 - 02:09 AM

I usually use ~10ng of Et-Br for every ml of TAE.

#3 sorcener

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Posted 11 March 2010 - 03:33 PM

Many Thanks!




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