2 replies to this topic

[sorcener]

Hi everybody!

  Just a small question, I want to reduce BrET use in my experiments and as we have only 1 microware used also for other people and things i prefer to use BrET on my TAE running buffer instead on gel.

  In your experience wich is the smallest concentration of BrET I can use on my buffer in order to have a good DNA view?

Thanks a lot!

[molgen]

I usually use ~10ng of Et-Br for every ml of TAE.

[sorcener]

Many Thanks!