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Ni-NTA his tag protein purification

Started by sud373, Jan 18 2010 02:21 AM

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#1 sud373

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Posted 18 January 2010 - 02:21 AM

hi
I want to purify my protein by using Ni-NTA cromatography tech. can any one provide me protocol for this?

thnx in advance
suddu

#2 KAUSHIK THAKKAR

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Posted 18 January 2010 - 04:26 AM

Hey,

This is asking for ready made stuff dear...!!
You should read first and ask queries for specific steps.


Anywayz, I will give it to you...here it is..

Let me know, if you have any doubts.

cheers

Kaushik

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