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Tips for antibody purification from crude serum?


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3 replies to this topic

#1 PhD

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Posted 18 January 2010 - 12:12 AM

Morning!

I was just wondering if anyone has any tips on what NOT to do during an antibody purification from crude serum? I found 2 protocols online and im going to try them out but since ive never done it before I was just wondering if maybe there are certain things that speed up or affect the quality etc etc

Thanks!

#2 aimikins

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Posted 20 January 2010 - 05:43 PM

dialyze, dialyze, dialyze...you don't want to muck up your column with teeny proteins you don't care about.
"it is a miracle that curiosity survives formal education" -A.E.

#3 mdfenko

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Posted 21 January 2010 - 09:34 AM

filter to remove any particulates (eg-denatured proteins).
talent does what it can
genius does what it must
i do what i get paid to do

#4 PhD

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Posted 25 January 2010 - 01:52 AM

Thanks!!




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