Morning!
I was just wondering if anyone has any tips on what NOT to do during an antibody purification from crude serum? I found 2 protocols online and im going to try them out but since ive never done it before I was just wondering if maybe there are certain things that speed up or affect the quality etc etc
Thanks!
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3 replies to this topic
#2
aimikins
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Sunflower
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Neutral
Neutral
Posted 20 January 2010 - 05:43 PM
dialyze, dialyze, dialyze...you don't want to muck up your column with teeny proteins you don't care about.
"it is a miracle that curiosity survives formal education" -A.E.
#3
mdfenko
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an elder
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Excellent
Excellent
Posted 21 January 2010 - 09:34 AM
filter to remove any particulates (eg-denatured proteins).
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
