Posted 17 January 2010 - 09:17 PM
I'll really appreciate any answers from you that can help me with my work, thank you a lot
Posted 18 January 2010 - 04:45 PM
If you only have a small number of cells, put them into a smaller flask, plate of dish. Go down to 96 well plates if you have to.
Posted 18 January 2010 - 06:33 PM
Posted 19 January 2010 - 03:37 PM
It could be that the cells need an attachment factor, though the ATCC page doesn't mention anything about it. The ATCC says that the cells will rapidly lose the myoblasts if subcultured too densely - perhaps this happened to the tube(s) of cells that you are using, before they were frozen down.
Posted 21 January 2010 - 06:59 PM
I don't know if the cells I thawed were sub-cultured densely or not, I got them from our lab library, others have frozen them a while ago.
My first cells died, now I'm culturing others ( the same line H9C2) and I'm having the same problem, cells are small and their growth is so slow, I reduced the surface area of culture, last time I used T30 flasks, now I'm culturing them in one well of 6-wells plate.
I hope this will work, I will let you know the result later
Thank you again for your help, I really appreciate it