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Methylation specific DMSO PCR


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#1 Linda2010

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Posted 17 January 2010 - 05:56 AM

Hello,

Does anyone here using for methylation analysis Methylation specific DMSO PCR, described http://jmd.amjpathol...nt/full/9/5/574

We are starting but nothing works.
And in anycase one important question if there are used DMSO I suppose from SIGMA it have to be 100% and if in reaction there are said that is used 8% DMSO it means that 8% of al PCR mix have to be DMSO ?

Thank You a lot!

#2 Adrian K

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Posted 17 January 2010 - 06:34 PM

Meaning of 8% DMSO is the final volume of your master mix in your PCR tube (lets say 25ul), there is 8% DMSO (2ul).
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