Hello,
Does anyone here using for methylation analysis Methylation specific DMSO PCR, described http://jmd.amjpathol...nt/full/9/5/574
We are starting but nothing works.
And in anycase one important question if there are used DMSO I suppose from SIGMA it have to be 100% and if in reaction there are said that is used 8% DMSO it means that 8% of al PCR mix have to be DMSO ?
Thank You a lot!
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Adrian K
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Posted 17 January 2010 - 06:34 PM
Meaning of 8% DMSO is the final volume of your master mix in your PCR tube (lets say 25ul), there is 8% DMSO (2ul).
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
