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Protein/Enzyme Stability at -80 degrees


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#1 KAUSHIK THAKKAR

KAUSHIK THAKKAR

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Posted 17 January 2010 - 04:43 AM

Hello Everybody,
I am working on a enzyme and its substrate. The enzyme is in the form of a membrane preparation (Sf9 ER fraction) and is resuspended in Hepes-NaOH (pH = 7.0) after ultracentrifugation. The substrate is expressed in E. coli, purified by Ni-NTA chromatography, and finally concentrated and resuspended in Hepes-NaOH.

I wanted to preserve both the enzyme and substrate at -80 degrees, I wanted to know if it is necessary to add glycerol as a cryoprotectant. The reason why I am asking this is that when I want to actually use it for the assay, the glycerol might inhibit the assay?

If I am to store it a 4 degrees, how long can I store it without glycerol?
:rolleyes:

Regards,
Kaushik
:)




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