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monoclonl antibody application specifity


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#1 sara.r

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Posted 16 January 2010 - 06:12 AM

Hi
how can antibodies become specific for an application for example western blotting or immunopercipitation?
what if I use an antibody that specified for WB by supplier in other application like elisa?
regards

#2 Gerard

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Posted 16 January 2010 - 08:02 AM

Antibodies don't become specific for an application, what it means is that the vendor has tested it for that application and garantees that it is suitable for that application.
Most of the times it will be suitable for other applications but it's something you've the figger it out yourself.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".




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