I would like to transduce my cells with lentivirus coding GFP. So letís assume I have a copGFP lentiviral particles bought from SantaCruz (sc-108084), they sell ready to use particles at 10^6 IFU. So assuming I need 2 MOI to infect my cells, 10^6 IFU is enough to infect 5*10^5 cells. Is this correct? The problem is that according to supplier it is enough to make 10-20 transductions, but in this case it is only for 5*10^4 cells/transduction. Are my calculations ok?
IFU, MOI, lentiviral vectors
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