3 replies to this topic

[Iker]

Hi:

I have to sterilizate a solution of DMEM with a polisacharide dissolved. The filtration (0,22uM) is not work. There is another method to sterilizate a culture medium?. Is posible pasteurizate the medium (60ºC 30 min) and after add the antibiotics, the L-glutamine and the serum?

Thanks
Iker

[Curtis]

0.22 um filteration is sufficient, why are you worried?

[microgirl]

What do you mean the 0.22 um filtration doesn't work? Will it not go thru the filter or is it not sterile? You could try filtering it successively thru a 0.8 then an 0.45 then an 0.22 filter.

[molgen]

Do you mean that polysaccharide clogs the filter?
If so, try preparing it in a sterile manner. If you got the polysaccharide from sigma then it comes sterile. Open it in a laminar flow and use sterile water do dissolve it. The just add it into the DMEM.