Hi everyone
I'm doing cloning and was wondering whether I can store the digested (with restriction enzymes) DNA before ligation. Does stored digested DNA inhibit ligation in any way? Any help please!
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3 replies to this topic
#1
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Enthusiast
Posted 15 January 2010 - 04:19 AM
We can find answers following our own ideas.
#2
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Veteran
Posted 15 January 2010 - 04:58 AM
The Question, on Jan 15 2010, 06:49 PM, said:
Hi everyone
I'm doing cloning and was wondering whether I can store the digested (with restriction enzymes) DNA before ligation. Does stored digested DNA inhibit ligation in any way? Any help please!
I'm doing cloning and was wondering whether I can store the digested (with restriction enzymes) DNA before ligation. Does stored digested DNA inhibit ligation in any way? Any help please!
yeah, i have tried doing that and it woks....although, i used up the digested product within a week of preparation..............i dunno if its advisable to store for longer than that!
#3
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Metaller Scientist
Posted 15 January 2010 - 05:01 AM
my friends told me keeping at -20 before ligation should be OK
#4
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Enthusiast
Posted 15 January 2010 - 05:16 AM
Curtis, on Jan 15 2010, 02:01 PM, said:
my friends told me keeping at -20 before ligation should be OK
Thanks a lot Veteran and Curtis.
I'll store it at -20 degree today and continue on Monday.
Thaks a lot again.
We can find answers following our own ideas.
