protein concentration factor
Posted 15 January 2010 - 02:17 AM
what is the effect of putting too much protein in enzyme assay? it is good to put as much protein as possible to make sure the reaction is complete. why we need to optimize and choose from the optimization.
Posted 15 January 2010 - 05:23 AM
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Posted 15 January 2010 - 07:38 AM
Posted 15 January 2010 - 08:09 AM
I would check the specific activity of your enzyme under the conditions that other labs measure it. If its extremely low, say 10-fold less or more than others, than your preparation of enzyme extract maybe bad, and you should make another preparation.
Another possibility is that your substrate is of poor quality. Maybe try to get another sample from a different company.