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RCAS virus infection


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3 replies to this topic

#1 sahil

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Posted 15 January 2010 - 12:15 AM

Dear all ,
I am using Replication competent avian virus (RCAS) as a tool to deliver my transgene . I made RCAS-GFP and checked the titer , titer was 5*10(power 7), now when I am trying to inject it into chicken at stage 10 ,after 24 hrs there is no GFP expression visible .
can anyone tell me what would be the problem in this case . was titer went down ?
please reply me

Regards
Sahil

#2 scigars

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Posted 15 January 2010 - 07:11 AM

bad... really bad

#3 scigars

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Posted 15 January 2010 - 07:12 AM

and yes: "titer" is down

#4 sahil

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Posted 16 January 2010 - 12:49 AM

can you please tell me ... what are the reasons behind this .. because all aliquots were frozen in -80 degree .
:o




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