One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.
Sean
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#2
Vini
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Posted 14 January 2010 - 10:42 AM
seanisaken, on Jan 15 2010, 12:46 AM, said:
One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.
Sean
Sean
ligation cannot occur b/w incompatible sites..........e.coli will not do it for you.
#3
seanisaken
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Posted 14 January 2010 - 11:11 AM
DRN, on Jan 14 2010, 10:42 AM, said:
seanisaken, on Jan 15 2010, 12:46 AM, said:
One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.
Sean
Sean
ligation cannot occur b/w incompatible sites..........e.coli will not do it for you.
Thank you, DRN. I will proceed to order some linkers then.
