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Incompatible restriction sites


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#1 seanisaken

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Posted 14 January 2010 - 10:16 AM

One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.

Sean

#2 Vini

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Posted 14 January 2010 - 10:42 AM

One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.

Sean



ligation cannot occur b/w incompatible sites..........e.coli will not do it for you.

#3 seanisaken

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Posted 14 January 2010 - 11:11 AM

One of the restrictions sites of my fragment is common with that of the vector, but not the other. Can I still transform competent cells with this incompletely ligated molecule? Will E.coli ligate the other half for me? I am just not sure about this. Thank you.

Sean



ligation cannot occur b/w incompatible sites..........e.coli will not do it for you.


Thank you, DRN. I will proceed to order some linkers then.




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