4 replies to this topic

[luc]

Hi,

I'm new to microRNA.I want to confirm the deep sequencing results.qrt-pcr and northern blot ,which way is better?

Thanks

[Fizban]

luc, on Jan 14 2010, 02:09 PM, said:

Hi,

I'm new to microRNA.I want to confirm the deep sequencing results.qrt-pcr and northern blot ,which way is better?

Thanks

i think qRT-PCR is easier, quicker and "multiplexable"!
one more thing: sensitive!
keep in mind that most of the miRNAs that you can see by qRT-PCR or sequencing WON'T show in northern blot.
that's my opinion
Fizban

[jessi]

Northern blot---but only if your reads from your library are >10. If few reads they may still show up on the northern blot.

Jessi

[Functional Screens]

qRT-PCR with Taqman probe

[Denny]

Functional Screens, on Mar 12 2010, 08:43 PM, said:

qRT-PCR with Taqman probe

Ditto