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qrt-pcr and northern blot ,which way is better?


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4 replies to this topic

#1 luc

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Posted 14 January 2010 - 05:09 AM

Hi,

I'm new to microRNA.I want to confirm the deep sequencing results.qrt-pcr and northern blot ,which way is better?

Thanks

#2 Fizban

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Posted 14 January 2010 - 05:39 AM

Hi,

I'm new to microRNA.I want to confirm the deep sequencing results.qrt-pcr and northern blot ,which way is better?

Thanks


i think qRT-PCR is easier, quicker and "multiplexable"!
one more thing: sensitive!
keep in mind that most of the miRNAs that you can see by qRT-PCR or sequencing WON'T show in northern blot.
that's my opinion
Fizban :blink:

#3 jessi

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Posted 04 March 2010 - 05:08 PM

Northern blot---but only if your reads from your library are >10. If few reads they may still show up on the northern blot.

Jessi

#4 Functional Screens

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Posted 12 March 2010 - 05:43 PM

qRT-PCR with Taqman probe

#5 Denny

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Posted 13 March 2010 - 09:04 AM

qRT-PCR with Taqman probe


Ditto




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