Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

0

tissue disruption help...

Started by mcmefferson, Jan 14 2010 03:07 AM

Please log in to reply

2 replies to this topic

#1 mcmefferson

member

Members
1 posts

0
Neutral

Posted 14 January 2010 - 03:07 AM

please help...I am looking for beads that possibly have blades or sharp edges so thay can cut as opposed to mash the tissue, I am working with living skin equivs (LSEs) and some real human skin...any ideas of where I can purchase anything like this pleeeeeease....many thanks in advance...

Back to top

#2 gogreen

Somewhere I belong

Active Members
90 posts

0
Neutral

Posted 14 January 2010 - 05:19 AM

Can I ask why u want to cut them down instead of grinding them !!? I used to use beads for isolating RNA from human skin (was using mortar and pestle before the lab got a beadmill...was a real PITA) never worked with LSE

Back to top

#3 bob1

Thelymitra pulchella

Global Moderators
4,350 posts

224
Excellent

Posted 14 January 2010 - 03:18 PM

mcmefferson, on Jan 14 2010, 04:07 AM, said:

please help...I am looking for beads that possibly have blades or sharp edges so thay can cut as opposed to mash the tissue, I am working with living skin equivs (LSEs) and some real human skin...any ideas of where I can purchase anything like this pleeeeeease....many thanks in advance...

If you are looking to get out live cells, most people just chop the tissue into small chunks and then digest in collagenase or trypsin for a while, then plate out the cells and don't disturb for a week or so, giving the cells some time to migrate out of the tissue pieces.