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PCR troubleshoooooting


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#1 maxmix

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Posted 13 January 2010 - 10:19 PM

Hi all,,Iam amplifying specific gen by PCR, I got the product last time ,,but know i got two band , i think of the primer dimer,,what is the solution? is it contamination issue? :blink:

#2 Vini

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Posted 14 January 2010 - 03:17 AM

Hi all,,Iam amplifying specific gen by PCR, I got the product last time ,,but know i got two band , i think of the primer dimer,,what is the solution? is it contamination issue? :blink:



Hey, it would be better if you also put the gel pic. need more details. What is the size of the other band?? maybe, last time u did the PCR, the extra band was there, running close to ur desired product and u didnt resolve it well enough (and therefore it appeared as a single band)....




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