Modifying collagen stiffness/rigidity in 3d cell culture
Can someone recommend a protocol to plate cells in collagen and modify the stiffness / rigidity of the collagen when plating or eaven better later - during cell culture?
Would help me a lot...
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Crazy4Cells
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Posted 17 February 2010 - 07:24 AM
Felina, on Jan 13 2010, 02:28 PM, said:
Modifying collagen stiffness/rigidity in 3d cell culture
Can someone recommend a protocol to plate cells in collagen and modify the stiffness / rigidity of the collagen when plating or eaven better later - during cell culture?
Would help me a lot...
Can someone recommend a protocol to plate cells in collagen and modify the stiffness / rigidity of the collagen when plating or eaven better later - during cell culture?
Would help me a lot...
I think your best bet is to search for papers/protocols that focus on crosslinking collagen. I did a quick keyword search on 3dcellculture.com and came across this paper that will hopefully address your question. You could also consider adding a second matrix material to your collagen to obtain the structural properties that you desire.
http://www.3dcellcul...Results/id/6819
I use that website all the time to find papers that apply to my research. With a few more details (like cell type), you can search the site for a list of papers that apply to your research exactly. I hope that paper helps, good luck!
