Dual Luciferase Assay
Posted 13 January 2010 - 09:04 AM
Can someone suggest me a ratio for the co-reporter vectors to be added to the transfection mix.
Posted 13 January 2010 - 09:13 AM
Posted 14 January 2010 - 12:23 PM
In addition to the good advice StevieRay has provided, it is also important to make sure that the presence of the Renilla control is not altering the expression of the firefly (usually the experimental) vector. In general you just need enough Renilla expression to be consistenly above background. The Dual-Luciferase Assay chemistry will quench the firefly signal 10,000-fold, so this more has to do with maintaining more normal biology than any concenr over the signals. I think you may find an article I co-wrote useful: http://www.promega.c...17/cn017_09.htm. See figure 3 especially. I hope this helps answer your question. Also, please always feel free to contact Promega Techncal Services by phone, e-mail, or chat.