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Do my antibodies need to stay sterile?


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5 replies to this topic

#1 Marilyn Haas

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Posted 13 January 2010 - 07:37 AM

How important is it for my antibodies to stay sterile if I am not using the cells for any downstream applications?

#2 HomeBrew

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Posted 13 January 2010 - 03:34 PM

Any organism contaminating your antibody stock solution will likely produce proteases that could destroy your antibodies. Is this what you mean?

#3 Marilyn Haas

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Posted 14 January 2010 - 07:59 AM

Any organism contaminating your antibody stock solution will likely produce proteases that will destroy your antibodies. Is this what you mean?



Yes thank you. I perform FACS regularly and recently I moved out from under a laminar flow hood to do my FACS at a bench closer to a new centrifuge to make the process quicker. I wanted to know if adding my antibodies outside the hood could cause problems.

#4 bob1

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Posted 14 January 2010 - 03:34 PM

I have very rarely had problems with antibody degradation through contamination (not doing FACS though) and I never use my Abs in a hood.

#5 lab rat

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Posted 15 January 2010 - 07:36 AM

We keep our stocks/hybridoma supernates sterile, and aliquot a working vial for bench work.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#6 Prep!

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Posted 15 January 2010 - 07:19 PM

yeah me too never used the hood for either reconstituting or diluting antibodies and have had no problems till now!!! i don do FSCS though... er reconstitute the antibody and aliquot into smaller vaolumes and store it in -20 until use... once we take out an aliquot as a working solution we dont freeze it further and make sure u dont keep the antibodies in a auto defrost freezer... the freeze thawing cycles will degrade the antibody
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