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recombinant protein quantification by flow cytometry

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#1 sara.r



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Posted 13 January 2010 - 02:11 AM

I have a elementary question, I am new in protein quantification, I expressed some proteins in CHO cells and extablish different clones now I need to compare expression level of my protein between different clones and chose the one with highest expression, I know different techniqus like elisa western blotting anf flow cytometry are used in this regard, but I dont know which is more accurate and more sensetive
because my expression level is probably very low.

thanks in advance

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