2 replies to this topic

[biobio]

Hi all,


I recently used the iFect reagent to knock-down a gene in vivo using intrathecal administration in the spinal cord. For those familiar with iFect, they also send you their own siRNA diluent. Thing is I had already diluted my siRNAs with water, so I used this to form complexes with iFect.

My experiment worked in terms of getting an effect (haven't evaluated knock down yet). My gut instict says it shouldn't really make a difference, but I am still wondering whether using water instead of their diluent to resuspend the siRNAs could decrease transfection efficiency.

I tried contacting Neuromics to ask and the response I got back was 'Dear customer, I would use the diluent'. Hardly helpful..


Thoughts, ideas or experiences please?

Edited by biobio, 12 January 2010 - 07:43 PM.

[miRNA man]

The response might have been from customer support - maybe you can try getting a hold of one of the R&D scientists in the company who knows the product inside out? Would be easier than testing it yourself!

[biobio]

miRNA man, on Jan 13 2010, 06:35 AM, said:

The response might have been from customer support - maybe you can try getting a hold of one of the R&D scientists in the company who knows the product inside out? Would be easier than testing it yourself!

I just kept asking and finally they sent me this:


''Experiment will work fine.

My expert says:

No, the customer does not have to use the diluent.

However, they could add their water-suspended siRNA to the diluent.

Either way, I do not think it will make much difference.''



So it's sorted! Thanks a lot for your answers guys!