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ethanol precipitation


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#1 jun_mol_bio

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Posted 12 January 2010 - 06:20 PM

hello everyone..

im trying to extract DNA from mammalian cell line to b specific normal NP cell line. the problem is after adding absolute ethanol the white stringy thing (DNA) is visible but i could not manage to pellet it down even though i spun it at 14000rpm for 10 min... to overcome this i tried overnight incubation in -20 degree Celsius but i still could not pellet it down. what could be causing this problem? any solution / suggestion for this?

#2 pDNA

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Posted 13 January 2010 - 09:56 AM

you can "spool" it out with a toothpick or something similar instead of centrifugation!

Regards,
p




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