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Question on wired PCR

Started by iseeyou, Jan 12 2010 11:16 AM

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#1 iseeyou

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Posted 12 January 2010 - 11:16 AM

Dear all,
I am here to ask a strange PCR result? I do one multi-plex PCR with bioline enzyme. I use 94 degree to denature the DNA. The results I got is very strange. I always can see a big smear smear from the DNA loading well to DNA ladder 2 kb position. I don't how this things happens.
Maybe some one you here can give me some adivce to give ride of it. I have used the same template to do another PCR and the results are very specific.
Thanks you all in advance and happy new year.

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#2 Adrian K

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Posted 12 January 2010 - 05:38 PM

Do you experience such incident when individual primer pairs from the multiplex were used?

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