I'm fractionating brain tissue to look at nuclear pools of a particular protein. I want to be sure my fractions are clean, specifically rid of plasma membrane bound protein pools which may cloud small changes in the nuclear pool. Do you know if Na/K ATPase will serve as a good marker to detect contamination of the nuclear fraction? I'm not sure whether I would expect ATPase from the nuclear membrane to be present...
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Na/K ATPase as marker for plasma membrane?
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