I found that my proteins which contain a S-S bond shown larger band size in reduced gel (128kDa) than non-reduced gel (109kDa), although there are more bands appeared in reduced gel. I repeated a few times but still got the same pattern. Shouldn't it show smaller band size in reduced gel? Any opinion or explanation for these results? Appreciate any help.
Edited by ABJF, 10 January 2010 - 06:20 PM.