role of NaCl in lysis buffer
Posted 08 January 2010 - 08:57 PM
I'm wondering the role of NaCl in lysis buffer for western. Can it be substituted by other salts>?
Posted 11 January 2010 - 09:21 AM
you could replace it with kcl but the sds will precipitate after adding sample buffer (kds is insoluble). and potassium salts may have a different effect on some of the proteins.
you could use licl but that is a lot more expensive.
keep in mind that the most abundant physiological salt is nacl.
Edited by mdfenko, 11 January 2010 - 09:22 AM.
genius does what it must
i do what i get paid to do