Let's also assume we have molecule#1 (reverse complement): 5'-TTTTCCCCGTGTGTGTGTGT-3'
And molecule #2 (complement): 5'-TGTGTGTGTGTGCCCCTTTT-3'
My question.. If we put mRNA together with one of the #1 or #2, in which cases will there be a DNA-RNA hybrid? Will it bind only with #1 or with both? In other words, does the orientation of the complement molecule matter and why?
I used to think both will bind because I was thinking of hydrogen bonds between bases only. But is there more to it?
Edited by biobio, 08 January 2010 - 06:32 PM.