I did miniprep for GABAA receptor after (fresh) transfrmation subunit and I got these bands.
Earlier any year before when I used to do the minipreps, I used to get the bands near 6000 bp.
Now I did the transformation with that same sample but i am getting the bands on the top of 1kb ladder.
What do you think that during this one year the plasmid DNA circular form has changed in to Nicked one?
Or I am loosing the correct plasmid.. I have done it almost 20 times but I never got that 6000 bp plasmid recently.
Now I am digesting it with restriction enzyme to see if ITS CUT OR NOT.
wHAT DO YOU THINK OF THIS. pICTURE OF THE GEL ATTACHED.