Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

IHC with anti-FLAG Ab

  • Please log in to reply
1 reply to this topic

#1 Rsm


    Post Dog

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 385 posts

Posted 08 January 2010 - 02:45 AM


I need to do an IHC on frozen tissue (skin) and detect a FLAG-tagged nuclear protein. I have tried Sigma M2 and F7425, no staining... Unfortunately, I don't have a positive control. Western does not work, too few cells (2000/mouse).
Does anyone know a good antibody or protocol? How about antigen retrieval, would that be better?
Any help/suggestions really appreciated!


Edited by Minna, 08 January 2010 - 02:47 AM.

I got soul, but I'm not a soldier

#2 doxorubicin



  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 193 posts

Posted 01 April 2010 - 08:46 PM

"FLAG" is a trademark belonging to sigma, so no other company should be able to sell an antibody with that name. Other companies sell their own version of the antibody recognizing the "FLAG" peptide: DYKDDDDK

Try doing some parallel experiments (Western and immunocytochemistry) with some cultured cells transfected with a plasmid expressing your protein. At least then you can be sure your reagents are working.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.