I need to do an IHC on frozen tissue (skin) and detect a FLAG-tagged nuclear protein. I have tried Sigma M2 and F7425, no staining... Unfortunately, I don't have a positive control. Western does not work, too few cells (2000/mouse).
Does anyone know a good antibody or protocol? How about antigen retrieval, would that be better?
Any help/suggestions really appreciated!
Edited by Minna, 08 January 2010 - 02:47 AM.