Hi all,
I want to run single strand DNA (the products of primer extension experiments) in a denaturing polyacrylamide gel using a miniprotean system (Biorad) (I am trying to avoid using a sequencing gel). The size of the DNAs is 60 and 160 nucleotides. I could adjust the concentration of acrylamide to resolve these fragments, but I am worried about the temperature to keep the denaturing conditions. Does anyone have a protocol to do this or some advice?
Thanks in advance
0
No replies to this topic
