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brain tissue fractionation (cytosolic and nuclear) protocol needed

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#1 yoshimi79



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Posted 07 January 2010 - 09:29 AM

Does anyone have a good protocol for preparation of nuclear and cytosolic proteins from mouse brain tissue? I'm looking at a protein present in high levels at the membrane, so I'm especially interested in the purity of the nuclear fraction (ie. membrane protein contaminating the nuclear fraction).

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