hello, i will be culturing neurons and macrophages together in a mixed culture. does anyone know of any methods for counting one cell type alone?
0
1 reply to this topic
#2
gfischer
-
- Global Moderators
-
- 179 posts
Veteran
7
Neutral
Neutral
Posted 07 January 2010 - 10:02 AM
prof. moriarty, on Jan 7 2010, 12:08 AM, said:
hello, i will be culturing neurons and macrophages together in a mixed culture. does anyone know of any methods for counting one cell type alone?
If you're counting manually, you should probably be able to differentiate the two populations by morphology. If you want to automate the process, you could use FACS with antibodies specific for the populations.
Above all things, if kindness is your king,
then heaven will be yours, before you meet your end
then heaven will be yours, before you meet your end
