2 replies to this topic

[bhulku]

I have inoculated a colony from 6 months old LB Amp Plate (store at 4C)  with wild type E.coli cells into 3ml of LB broth. I have incubated it overnight 18hrs, In spite of over night incubation the OD600 was 1.95 and 0.5 in two different 3ml cultures.

So do you think that the 6 month old cells might be the reason for slow growth. For how long the streaked plate will stay good at 4C?

Thanks.

[jangajarn]

try restreaking the colony onto fresh plates and then grow them in the broth

[phage434]

I think whatever is growing bears only accidental relationship to what was originally on that plate.