immunofluorescence for HUVEC
Posted 05 January 2010 - 07:11 PM
i am new to cell culture.my boss told to do immunofluorescence but i am facing problem in fixing the HUVEC cells.i used normal procedure done by others in paper.but when i going to fix the cells the cells are shrinking.so kindly help me how to get rid of this.
Is anybody having detail protocol for immunofluorescence for HUVEC cells.
thanks in advance,
Posted 06 January 2010 - 03:13 PM
Posted 07 January 2010 - 06:10 PM
as soon as i add acteone/methanol on coverslip the cells started to shrink and looses its shape.so could not proceed further.
if u have any good paper where they have used HUVEC CELLS experiment...
Posted 10 January 2010 - 04:03 PM