Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Preparing ethidium bromide stock solution.

  • Please log in to reply
2 replies to this topic

#1 spellberg56



  • Active Members
  • Pip
  • 13 posts

Posted 05 January 2010 - 02:12 PM

Hello, I need help preparing ethidium bromide stock solution. I have the powder but the concentration is supposed to be 10 ug/ml and I can't measure out that small of a volume plus the powder has a clumpy consistency which makes it hard to work with in the first place. Can someone tell me how to make this working solution?

Thank you

#2 phage434



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,747 posts

Posted 05 January 2010 - 02:32 PM

The usual stock solution concentration of EtBr is 10 mg/ml, or 1000x higher. Are you sure you need the lower concentration?
I would make this by first making a 10 mg/ml stock and diluting 1000x (assuming that this is what you really need).

#3 Mazhar Hussain

Mazhar Hussain


  • Active Members
  • Pip
  • 17 posts

Posted 08 January 2010 - 05:54 AM

By default,
EtBr is actually 10mg per ml
we used to convert it to .5 microgram per ml
Mazhar Hussain
Microbiology & Molecular Genetics
Website: Microbiology On-Line
FaceBook Group: Microbiology On-Line

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.