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Preparing ethidium bromide stock solution.


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#1 spellberg56

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Posted 05 January 2010 - 02:12 PM

Hello, I need help preparing ethidium bromide stock solution. I have the powder but the concentration is supposed to be 10 ug/ml and I can't measure out that small of a volume plus the powder has a clumpy consistency which makes it hard to work with in the first place. Can someone tell me how to make this working solution?

Thank you

#2 phage434

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Posted 05 January 2010 - 02:32 PM

The usual stock solution concentration of EtBr is 10 mg/ml, or 1000x higher. Are you sure you need the lower concentration?
I would make this by first making a 10 mg/ml stock and diluting 1000x (assuming that this is what you really need).

#3 Mazhar Hussain

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Posted 08 January 2010 - 05:54 AM

By default,
EtBr is actually 10mg per ml
we used to convert it to .5 microgram per ml
Mazhar Hussain
Microbiology & Molecular Genetics
Website: Microbiology On-Line
FaceBook Group: Microbiology On-Line




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