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software for plasmid drawing


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12 replies to this topic

#1 lufang

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Posted 05 January 2010 - 11:49 AM

For the publication, the plasmid should be drawn as EPS format. i'd like to ask which kind of software usually is used for this purpose? Thanks in advance.

#2 pcrman

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Posted 05 January 2010 - 12:26 PM

You can export vector map from Vector NTI to adobe illustrator, the most sophisticated vector graphics editor.

#3 lufang

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Posted 06 January 2010 - 12:13 AM

i didn't have this software :P

#4 Mazhar Hussain

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Posted 08 January 2010 - 05:42 AM

i didn't have this software :)

Dear!
No software is required...
U can go for this link...
Paste your sequence and Draw Plasmid map

http://wishart.biolo...pper/index.html
Mazhar Hussain
Microbiology & Molecular Genetics
Website: Microbiology On-Line
FaceBook Group: Microbiology On-Line

#5 molgen

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Posted 10 January 2010 - 04:52 AM

You can use pDRAW32 or GENtle or plasmid processor.
All of wich are freeware

Edited by molgen, 10 January 2010 - 04:53 AM.


#6 peng_sand

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Posted 01 February 2010 - 03:40 AM

hi friends,
I am new to R.I would like to know R-PLUS.Does any know where can I get the free training for R-PLUS.

Regards,
Peng.

#7 bglick

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Posted 27 April 2012 - 02:48 PM

Try SnapGene Viewer:

http://www.snapgene....napgene_viewer/

It's free, and you can export the plasmid maps in many different formats.

#8 bob1

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Posted 27 April 2012 - 06:29 PM

I use ApE (a plasmid editor) freeware from here to make the images and then export in eps format.

#9 lyok

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Posted 23 November 2012 - 11:59 AM

I use ApE (a plasmid editor) freeware from here to make the images and then export in eps format.


Dear bob1 do you know how you can simply enter a "fake" plasmid with only certain restriction sites/genes?
For example: I only know 3 genes that are on the plasmid and 10 restriction enzymes , for the rest I know nothing about the sequence of the plasmid itself.
So how can I create this kind of plasmid? I cant seem to find how this works.

#10 bob1

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Posted 23 November 2012 - 11:41 PM

Just get random sequence from anywhere (even create it yourself by using repeats) make sure that it is roughly the right size. Add your genes of interest/restriction sites, select them and then add them as features or use the restriction site tool to highlight the RE sites then draw the map...

#11 lyok

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Posted 24 November 2012 - 12:35 AM

Just get random sequence from anywhere (even create it yourself by using repeats) make sure that it is roughly the right size. Add your genes of interest/restriction sites, select them and then add them as features or use the restriction site tool to highlight the RE sites then draw the map...


ok, I see.
There is no option thus to generate random sequence with ape itself then.

I'll do it like that.

#12 memari

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Posted 24 November 2012 - 04:53 PM

http://sites.google....eebookmarksorg/


http://babakmemari.w...lysis-software/

http://www.mybiosoftware.com/
-----
Babak Memari

#13 branthackett

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Posted 19 September 2014 - 08:42 AM

Gene Construction Kit® (GCK) is a useful tool for plasmid mapping, cloning simulation, and DNA analysis. It has been consistently reviewed as being one of the easiest to use applications for simulating multi-step cloning operations, and offers extensive flexibility in the graphical formatting of DNA sequences - and - generating those colorful, customized plasmid maps seen in publications (sample attached). You can easily copy and paste grapical maps from GCK to image editing programs, or even create hi-res PDF (Mac) or XPS (Win) exports from within the program. It's been around since the mid-90's, and was one of, if not "the very first software" for performing these types of graphical manipulations of DNA sequence data.

 

More information, and a free demo version is available from the website: http://www.textco.com

 

Check it out!

Attached Thumbnails

  • Gene Construction Kit Sample.png

Edited by branthackett, 19 September 2014 - 08:42 AM.





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