software for plasmid drawing
Posted 05 January 2010 - 11:49 AM
Posted 05 January 2010 - 12:26 PM
Posted 08 January 2010 - 05:42 AM
i didn't have this software
No software is required...
U can go for this link...
Paste your sequence and Draw Plasmid map
Microbiology & Molecular Genetics
Website: Microbiology On-Line
FaceBook Group: Microbiology On-Line
Posted 01 February 2010 - 03:40 AM
I am new to R.I would like to know R-PLUS.Does any know where can I get the free training for R-PLUS.
Posted 23 November 2012 - 11:59 AM
I use ApE (a plasmid editor) freeware from here to make the images and then export in eps format.
Dear bob1 do you know how you can simply enter a "fake" plasmid with only certain restriction sites/genes?
For example: I only know 3 genes that are on the plasmid and 10 restriction enzymes , for the rest I know nothing about the sequence of the plasmid itself.
So how can I create this kind of plasmid? I cant seem to find how this works.
Posted 23 November 2012 - 11:41 PM
Posted 24 November 2012 - 12:35 AM
Just get random sequence from anywhere (even create it yourself by using repeats) make sure that it is roughly the right size. Add your genes of interest/restriction sites, select them and then add them as features or use the restriction site tool to highlight the RE sites then draw the map...
ok, I see.
There is no option thus to generate random sequence with ape itself then.
I'll do it like that.
Posted 19 September 2014 - 08:42 AM
Gene Construction Kit® (GCK) is a useful tool for plasmid mapping, cloning simulation, and DNA analysis. It has been consistently reviewed as being one of the easiest to use applications for simulating multi-step cloning operations, and offers extensive flexibility in the graphical formatting of DNA sequences - and - generating those colorful, customized plasmid maps seen in publications (sample attached). You can easily copy and paste grapical maps from GCK to image editing programs, or even create hi-res PDF (Mac) or XPS (Win) exports from within the program. It's been around since the mid-90's, and was one of, if not "the very first software" for performing these types of graphical manipulations of DNA sequence data.
More information, and a free demo version is available from the website: http://www.textco.com
Check it out!
Edited by branthackett, 19 September 2014 - 08:42 AM.