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software for plasmid drawing


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13 replies to this topic

#1 lufang

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Posted 05 January 2010 - 11:49 AM

For the publication, the plasmid should be drawn as EPS format. i'd like to ask which kind of software usually is used for this purpose? Thanks in advance.

#2 pcrman

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Posted 05 January 2010 - 12:26 PM

You can export vector map from Vector NTI to adobe illustrator, the most sophisticated vector graphics editor.

#3 lufang

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Posted 06 January 2010 - 12:13 AM

i didn't have this software :P

#4 Mazhar Hussain

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Posted 08 January 2010 - 05:42 AM

i didn't have this software :)

Dear!
No software is required...
U can go for this link...
Paste your sequence and Draw Plasmid map

http://wishart.biolo...pper/index.html
Mazhar Hussain
Microbiology & Molecular Genetics
Website: Microbiology On-Line
FaceBook Group: Microbiology On-Line

#5 molgen

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Posted 10 January 2010 - 04:52 AM

You can use pDRAW32 or GENtle or plasmid processor.
All of wich are freeware

Edited by molgen, 10 January 2010 - 04:53 AM.


#6 peng_sand

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Posted 01 February 2010 - 03:40 AM

hi friends,
I am new to R.I would like to know R-PLUS.Does any know where can I get the free training for R-PLUS.

Regards,
Peng.

#7 bglick

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Posted 27 April 2012 - 02:48 PM

Try SnapGene Viewer:

http://www.snapgene....napgene_viewer/

It's free, and you can export the plasmid maps in many different formats.

#8 bob1

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Posted 27 April 2012 - 06:29 PM

I use ApE (a plasmid editor) freeware from here to make the images and then export in eps format.

#9 lyok

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Posted 23 November 2012 - 11:59 AM

I use ApE (a plasmid editor) freeware from here to make the images and then export in eps format.


Dear bob1 do you know how you can simply enter a "fake" plasmid with only certain restriction sites/genes?
For example: I only know 3 genes that are on the plasmid and 10 restriction enzymes , for the rest I know nothing about the sequence of the plasmid itself.
So how can I create this kind of plasmid? I cant seem to find how this works.

#10 bob1

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Posted 23 November 2012 - 11:41 PM

Just get random sequence from anywhere (even create it yourself by using repeats) make sure that it is roughly the right size. Add your genes of interest/restriction sites, select them and then add them as features or use the restriction site tool to highlight the RE sites then draw the map...

#11 lyok

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Posted 24 November 2012 - 12:35 AM

Just get random sequence from anywhere (even create it yourself by using repeats) make sure that it is roughly the right size. Add your genes of interest/restriction sites, select them and then add them as features or use the restriction site tool to highlight the RE sites then draw the map...


ok, I see.
There is no option thus to generate random sequence with ape itself then.

I'll do it like that.

#12 BMF

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Posted 24 November 2012 - 04:53 PM

http://sites.google....eebookmarksorg/


http://babakmemari.w...lysis-software/

http://www.mybiosoftware.com/

#13 branthackett

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Posted 19 September 2014 - 08:42 AM

Gene Construction Kit® (GCK) is a useful tool for plasmid mapping, cloning simulation, and DNA analysis. It has been consistently reviewed as being one of the easiest to use applications for simulating multi-step cloning operations, and offers extensive flexibility in the graphical formatting of DNA sequences - and - generating those colorful, customized plasmid maps seen in publications (sample attached). You can easily copy and paste grapical maps from GCK to image editing programs, or even create hi-res PDF (Mac) or XPS (Win) exports from within the program. It's been around since the mid-90's, and was one of, if not "the very first software" for performing these types of graphical manipulations of DNA sequence data.

 

More information, and a free demo version is available from the website: http://www.textco.com

 

Check it out!

Attached Thumbnails

  • Gene Construction Kit Sample.png

Edited by branthackett, 19 September 2014 - 08:42 AM.


#14 vladCH

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Posted 02 April 2016 - 10:39 AM


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I use ApE (a plasmid editor) freeware from here to make the images and then export in eps format.


Dear bob1 do you know how you can simply enter a "fake" plasmid with only certain restriction sites/genes?
For example: I only know 3 genes that are on the plasmid and 10 restriction enzymes , for the rest I know nothing about the sequence of the plasmid itself.
So how can I create this kind of plasmid? I cant seem to find how this works.

 

Hi, you can generate a random sequence of the required length using Random Sequence Generator and paste it into the WebDSV DNA sequence editor. Then add some "mock" genes and restriction sites to the required positions and let the program generate a plasmid map (click the "map" button). Then you can right-click the map and save it as a png file.






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