11 replies to this topic

[lufang]

For the publication, the plasmid should be drawn as EPS format. i'd like to ask which kind of software usually is used for this purpose? Thanks in advance.

[pcrman]

You can export vector map from Vector NTI to adobe illustrator, the most sophisticated vector graphics editor.

[lufang]

i didn't have this software

[Mazhar Hussain]

lufang, on Jan 6 2010, 03:13 AM, said:

i didn't have this software

Dear!
No software is required...
U can go for this link...
Paste your sequence and Draw Plasmid map

http://wishart.biolo...pper/index.html

[molgen]

You can use pDRAW32 or GENtle or plasmid processor.
All of wich are freeware

Edited by molgen, 10 January 2010 - 04:53 AM.

[peng_sand]

hi friends,
    I am new to R.I would like to know R-PLUS.Does any know where can I get the free training for R-PLUS.

Regards,
Peng.

[bglick]

Try SnapGene Viewer:

http://www.snapgene....napgene_viewer/

It's free, and you can export the plasmid maps in many different formats.

[bob1]

I use ApE (a plasmid editor) freeware from here to make the images and then export in eps format.

[lyok]

bob1, on 27 April 2012 - 06:29 PM, said:

I use ApE (a plasmid editor) freeware from here to make the images and then export in eps format.

Dear bob1 do you know how you can simply enter a "fake" plasmid with only certain restriction sites/genes?
For example: I only know 3 genes that are on the plasmid and 10 restriction enzymes , for the rest I know nothing about the sequence of the plasmid itself.
So how can I create this kind of plasmid? I cant seem to find how this works.

[bob1]

Just get random sequence from anywhere (even create it yourself by using repeats) make sure that it is roughly the right size.  Add your genes of interest/restriction sites, select them and then add them as features or use the restriction site tool to highlight the RE sites then draw the map...

[lyok]

bob1, on 23 November 2012 - 11:41 PM, said:

Just get random sequence from anywhere (even create it yourself by using repeats) make sure that it is roughly the right size.  Add your genes of interest/restriction sites, select them and then add them as features or use the restriction site tool to highlight the RE sites then draw the map...

ok, I see.
There is no option thus to generate  random sequence with ape itself then.

I'll do it like that.

[memari]

http://sites.google....eebookmarksorg/


http://babakmemari.w...lysis-software/

http://www.mybiosoftware.com/